three-segmented dimer with internal symmetry and we identify conserved residues on the surface and within the dimerization interface that are required for the Rock-Shroom interaction and Shroom
نویسندگان
چکیده
Swarna Mohan, Ryan Rizaldy, Debamitra Das, Robert J. Bauer, Annie Heroux, Michael A. Trakselis, Jeffrey D. Hildebrand, and Andrew P. VanDemark. * Department of Biological Sciences, University of Pittsburgh, Pittsburgh PA 15260, USA Department of Chemistry, University of Pittsburgh, Pittsburgh PA 15260, USA Department of Biology, Brookhaven National Laboratory, Upton, NY 11973, USA Corresponding Authors Running Head: Structure of the Shroom-Domain 2
منابع مشابه
Structure of Shroom domain 2 reveals a three-segmented coiled-coil required for dimerization, Rock binding, and apical constriction
Shroom (Shrm) proteins are essential regulators of cell shape and tissue morpho-logy during animal development that function by interacting directly with the coiled-coil region of Rho kinase (Rock). The Shrm-Rock interaction is sufficient to direct Rock subcellular localization and the subsequent assembly of contractile actomyosin networks in defined subcellular locales. However, it is unclear ...
متن کاملStructure of a Highly Conserved Domain of Rock1 Required for Shroom-Mediated Regulation of Cell Morphology
Rho-associated coiled coil containing protein kinase (Rho-kinase or Rock) is a well-defined determinant of actin organization and dynamics in most animal cells characterized to date. One of the primary effectors of Rock is non-muscle myosin II. Activation of Rock results in increased contractility of myosin II and subsequent changes in actin architecture and cell morphology. The regulation of R...
متن کاملDifferential Actin-dependent Localization Modulates the Evolutionarily Conserved Activity of Shroom
Shroom is an actin-associated determinant of cell morphology that is required for neural tube closure in both mice and frogs. Shroom regulates this process by causing apical constriction of epithelial cells via a pathway involvingmyosin II.Herewe report on characterization of the Shroom-related proteins Apxl andKIAA1202 and their role in cell architecture. Shroom,Apxl, and KIAA1202 exhibit diff...
متن کاملShroom regulates epithelial cell shape via the apical positioning of an actomyosin network.
The actin-binding protein Shroom is essential for neural tube morphogenesis in multiple vertebrate organisms, indicating its function is evolutionarily conserved. Shroom facilitates neurulation by regulating the morphology of neurepithelial cells. Shroom localizes to the apical tip of adherens junctions of neural ectoderm cells in vivo and to the apical junctional complex (AJC) in MDCK cells. I...
متن کاملDifferential actin-dependent localization modulates the evolutionarily conserved activity of Shroom family proteins.
Shroom is an actin-associated determinant of cell morphology that is required for neural tube closure in both mice and frogs. Shroom regulates this process by causing apical constriction of epithelial cells via a pathway involving myosin II. Here we report on characterization of the Shroom-related proteins Apxl and KIAA1202 and their role in cell architecture. Shroom, Apxl, and KIAA1202 exhibit...
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تاریخ انتشار 2012